Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18.
Identifieur interne : 001D09 ( Main/Exploration ); précédent : 001D08; suivant : 001D10Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18.
Auteurs : Siqingaowa Suo [République populaire de Chine] ; Yudong Ren ; Guangxing Li ; Dante Zarlenga ; Ri-E Bu ; Dingding Su ; Xunliang Li ; Pengchong Li ; Fandan Meng ; Chao Wang ; Xiaofeng RenSource :
- Virus research [ 1872-7492 ] ; 2012.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Adjuvants immunologiques (génétique), Animaux, Anticorps antiviraux (sang), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Interféron gamma (métabolisme), Interleukine-18 (administration et posologie), Interleukine-18 (génétique), Interleukine-4 (métabolisme), Lymphocytes T (immunologie), Lymphocytes T cytotoxiques (immunologie), Plasmides, Prolifération cellulaire, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Rate (immunologie), Sang (immunologie), Souris, Vaccins à ADN (administration et posologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus de la diarrhée porcine épidémique (génétique), Virus de la diarrhée porcine épidémique (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques, Interleukine-18, Vaccins à ADN.
- génétique : Adjuvants immunologiques, Glycoprotéines membranaires, Interleukine-18, Protéines de l'enveloppe virale, Vaccins à ADN, Virus de la diarrhée porcine épidémique.
- immunologie : Glycoprotéines membranaires, Lymphocytes T, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Rate, Sang, Vaccins à ADN, Virus de la diarrhée porcine épidémique.
- métabolisme : Interféron gamma, Interleukine-4.
- sang : Anticorps antiviraux.
- Animaux, Glycoprotéine de spicule des coronavirus, Plasmides, Prolifération cellulaire, Souris.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Adjuvants, Immunologic (genetics), Animals, Antibodies, Viral (blood), Blood (immunology), Cell Proliferation, Interferon-gamma (metabolism), Interleukin-18 (administration & dosage), Interleukin-18 (genetics), Interleukin-4 (metabolism), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Plasmids, Porcine epidemic diarrhea virus (genetics), Porcine epidemic diarrhea virus (immunology), Spike Glycoprotein, Coronavirus, Spleen (immunology), T-Lymphocytes (immunology), T-Lymphocytes, Cytotoxic (immunology), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Interleukin-18, Vaccines, DNA.
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Adjuvants, Immunologic, Interleukin-18, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- genetics : Porcine epidemic diarrhea virus.
- immunology : Blood, Membrane Glycoproteins, Porcine epidemic diarrhea virus, Spleen, T-Lymphocytes, T-Lymphocytes, Cytotoxic, Vaccines, DNA, Viral Envelope Proteins.
- chemical , metabolism : Interferon-gamma, Interleukin-4.
- Animals, Cell Proliferation, Mice, Plasmids, Spike Glycoprotein, Coronavirus.
Abstract
Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, a highly contagious enteric disease of swine. The Spike (S) protein is one of the main structural proteins of PEDV capable of inducing neutralizing antibodies in vivo. Herein, we generated three distinct DNA constructs in the eukaryotic expression plasmid pVAX1; one encoding the S protein [pVAX1-(PEDV-S)], the second encoding the N-terminal fragment (S1) [pVAX1-(PEDV-S1)] containing potent antigenic sites, and the third expressing the porcine interleukin-18 (pIL-18) [pVAX1-(IL-18)]. Immunofluorescence assays in BHK-21 cells demonstrated successful protein expression from all 3 constructs. Kunming mice were injected separately with each of these constructs or with a pVAX1-(PEDV-S1)/pVAX1-(IL-18) combination, an attenuated PEDV vaccine, or vector only control. Animals were examined for T lymphocyte proliferation, anti-PEDV antibodies, IFN-γ and IL-4 protein levels, and cytotoxic T cell function in mouse peripheral blood and spleen. In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-(IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1).
DOI: 10.1016/j.virusres.2012.05.007
PubMed: 22643071
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001355
- to stream PubMed, to step Curation: 001355
- to stream PubMed, to step Checkpoint: 001340
- to stream Ncbi, to step Merge: 002511
- to stream Ncbi, to step Curation: 002511
- to stream Ncbi, to step Checkpoint: 002511
- to stream Main, to step Merge: 001D29
- to stream Main, to step Curation: 001D09
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18.</title><author><name sortKey="Suo, Siqingaowa" sort="Suo, Siqingaowa" uniqKey="Suo S" first="Siqingaowa" last="Suo">Siqingaowa Suo</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Yudong" sort="Ren, Yudong" uniqKey="Ren Y" first="Yudong" last="Ren">Yudong Ren</name></author><author><name sortKey="Li, Guangxing" sort="Li, Guangxing" uniqKey="Li G" first="Guangxing" last="Li">Guangxing Li</name></author><author><name sortKey="Zarlenga, Dante" sort="Zarlenga, Dante" uniqKey="Zarlenga D" first="Dante" last="Zarlenga">Dante Zarlenga</name></author><author><name sortKey="Bu, Ri E" sort="Bu, Ri E" uniqKey="Bu R" first="Ri-E" last="Bu">Ri-E Bu</name></author><author><name sortKey="Su, Dingding" sort="Su, Dingding" uniqKey="Su D" first="Dingding" last="Su">Dingding Su</name></author><author><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name></author><author><name sortKey="Li, Pengchong" sort="Li, Pengchong" uniqKey="Li P" first="Pengchong" last="Li">Pengchong Li</name></author><author><name sortKey="Meng, Fandan" sort="Meng, Fandan" uniqKey="Meng F" first="Fandan" last="Meng">Fandan Meng</name></author><author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name></author><author><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22643071</idno><idno type="pmid">22643071</idno><idno type="doi">10.1016/j.virusres.2012.05.007</idno><idno type="wicri:Area/PubMed/Corpus">001355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001355</idno><idno type="wicri:Area/PubMed/Curation">001355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001355</idno><idno type="wicri:Area/PubMed/Checkpoint">001340</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001340</idno><idno type="wicri:Area/Ncbi/Merge">002511</idno><idno type="wicri:Area/Ncbi/Curation">002511</idno><idno type="wicri:Area/Ncbi/Checkpoint">002511</idno><idno type="wicri:Area/Main/Merge">001D29</idno><idno type="wicri:Area/Main/Curation">001D09</idno><idno type="wicri:Area/Main/Exploration">001D09</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18.</title><author><name sortKey="Suo, Siqingaowa" sort="Suo, Siqingaowa" uniqKey="Suo S" first="Siqingaowa" last="Suo">Siqingaowa Suo</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Yudong" sort="Ren, Yudong" uniqKey="Ren Y" first="Yudong" last="Ren">Yudong Ren</name></author><author><name sortKey="Li, Guangxing" sort="Li, Guangxing" uniqKey="Li G" first="Guangxing" last="Li">Guangxing Li</name></author><author><name sortKey="Zarlenga, Dante" sort="Zarlenga, Dante" uniqKey="Zarlenga D" first="Dante" last="Zarlenga">Dante Zarlenga</name></author><author><name sortKey="Bu, Ri E" sort="Bu, Ri E" uniqKey="Bu R" first="Ri-E" last="Bu">Ri-E Bu</name></author><author><name sortKey="Su, Dingding" sort="Su, Dingding" uniqKey="Su D" first="Dingding" last="Su">Dingding Su</name></author><author><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name></author><author><name sortKey="Li, Pengchong" sort="Li, Pengchong" uniqKey="Li P" first="Pengchong" last="Li">Pengchong Li</name></author><author><name sortKey="Meng, Fandan" sort="Meng, Fandan" uniqKey="Meng F" first="Fandan" last="Meng">Fandan Meng</name></author><author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name></author><author><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name></author></analytic><series><title level="j">Virus research</title><idno type="eISSN">1872-7492</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term><term>Adjuvants, Immunologic (genetics)</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Blood (immunology)</term><term>Cell Proliferation</term><term>Interferon-gamma (metabolism)</term><term>Interleukin-18 (administration & dosage)</term><term>Interleukin-18 (genetics)</term><term>Interleukin-4 (metabolism)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Plasmids</term><term>Porcine epidemic diarrhea virus (genetics)</term><term>Porcine epidemic diarrhea virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spleen (immunology)</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term><term>Adjuvants immunologiques (génétique)</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Interféron gamma (métabolisme)</term><term>Interleukine-18 (administration et posologie)</term><term>Interleukine-18 (génétique)</term><term>Interleukine-4 (métabolisme)</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Plasmides</term><term>Prolifération cellulaire</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Rate (immunologie)</term><term>Sang (immunologie)</term><term>Souris</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus de la diarrhée porcine épidémique (génétique)</term><term>Virus de la diarrhée porcine épidémique (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Interleukin-18</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Interleukin-18</term><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Interleukine-18</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Porcine epidemic diarrhea virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Glycoprotéines membranaires</term><term>Interleukine-18</term><term>Protéines de l'enveloppe virale</term><term>Vaccins à ADN</term><term>Virus de la diarrhée porcine épidémique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Lymphocytes T</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Rate</term><term>Sang</term><term>Vaccins à ADN</term><term>Virus de la diarrhée porcine épidémique</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Blood</term><term>Membrane Glycoproteins</term><term>Porcine epidemic diarrhea virus</term><term>Spleen</term><term>T-Lymphocytes</term><term>T-Lymphocytes, Cytotoxic</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term><term>Interleukin-4</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term><term>Interleukine-4</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Proliferation</term><term>Mice</term><term>Plasmids</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Plasmides</term><term>Prolifération cellulaire</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, a highly contagious enteric disease of swine. The Spike (S) protein is one of the main structural proteins of PEDV capable of inducing neutralizing antibodies in vivo. Herein, we generated three distinct DNA constructs in the eukaryotic expression plasmid pVAX1; one encoding the S protein [pVAX1-(PEDV-S)], the second encoding the N-terminal fragment (S1) [pVAX1-(PEDV-S1)] containing potent antigenic sites, and the third expressing the porcine interleukin-18 (pIL-18) [pVAX1-(IL-18)]. Immunofluorescence assays in BHK-21 cells demonstrated successful protein expression from all 3 constructs. Kunming mice were injected separately with each of these constructs or with a pVAX1-(PEDV-S1)/pVAX1-(IL-18) combination, an attenuated PEDV vaccine, or vector only control. Animals were examined for T lymphocyte proliferation, anti-PEDV antibodies, IFN-γ and IL-4 protein levels, and cytotoxic T cell function in mouse peripheral blood and spleen. In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-(IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1).</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Bu, Ri E" sort="Bu, Ri E" uniqKey="Bu R" first="Ri-E" last="Bu">Ri-E Bu</name><name sortKey="Li, Guangxing" sort="Li, Guangxing" uniqKey="Li G" first="Guangxing" last="Li">Guangxing Li</name><name sortKey="Li, Pengchong" sort="Li, Pengchong" uniqKey="Li P" first="Pengchong" last="Li">Pengchong Li</name><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name><name sortKey="Meng, Fandan" sort="Meng, Fandan" uniqKey="Meng F" first="Fandan" last="Meng">Fandan Meng</name><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name><name sortKey="Ren, Yudong" sort="Ren, Yudong" uniqKey="Ren Y" first="Yudong" last="Ren">Yudong Ren</name><name sortKey="Su, Dingding" sort="Su, Dingding" uniqKey="Su D" first="Dingding" last="Su">Dingding Su</name><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name><name sortKey="Zarlenga, Dante" sort="Zarlenga, Dante" uniqKey="Zarlenga D" first="Dante" last="Zarlenga">Dante Zarlenga</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Suo, Siqingaowa" sort="Suo, Siqingaowa" uniqKey="Suo S" first="Siqingaowa" last="Suo">Siqingaowa Suo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22643071
|texte= Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22643071" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |